Notch1 antibody

Catalog No. ABIN807923

The Mouse Monoclonal anti-Notch1 antibody has been validated for WB, FACS, ICC, IP and IHC (fro). It is suitable to detect Notch1 in samples from Human and Mouse. There are 4+ publications available.

Quick Overview for Notch1 antibody (ABIN807923)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MN1A

Product Details anti-Notch1 Antibody

Purpose: Anti-Notch1 Purified

Specificity: The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

No Cross-Reactivity: Rat

Cross-Reactivity (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: GST fusion protein containing cdc10-NCR region of mouse Notch1

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Notch1 antibody (ABIN807923)

Khwaja, Liu, Tong, Jin, Pear, van Deursen, Bram: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, (2010) (PubMed).

Watanabe, Nagaoka, Lee, Bianco, Gonzales, Castro, Rangel, Sakamoto, Sun, Callahan, Salomon: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, (2009) (PubMed).

Sun, Li, Vercherat, Gulbagci, Acharjee, Li, Chung, Thin, Taneja: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, (2007) (PubMed).

Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, (2000) (PubMed).

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Notch1

Background: Notch receptor 1,Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.,AOS5, TAN1, hN1, AOVD1

Gene ID: 4851

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development