TRPV3 antibody (AA 458-474)

Catalog No. ABIN863127

Cited in 4+ publications. The Mouse Monoclonal anti-TRPV3 antibody (Clone N15-39 (Formerly S15-39)) (ABIN863127) specifically detects TRPV3 in WB, IHC, IP, ICC, IF and AA. The antibody is reactive with Rat samples.

Quick Overview for TRPV3 antibody (AA 458-474) (ABIN863127)

Target: TRPV3 (Transient Receptor Potential Cation Channel, Subfamily V, Member 3 (TRPV3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRPV3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Clone: N15-39 (Formerly S15-39)

Product Details anti-TRPV3 Antibody

Binding Specificity: AA 458-474

Specificity: Detects ~70 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863127 was sufficient for detection of TrpV3 in 10 μg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-TRPV3 antibody (ABIN863127)

Liebe, Liebe, Sponder, Mergler, Stumpff: "Effects of butyrate- on ruminal Ca2+ transport: evidence for the involvement of apically expressed TRPV3 and TRPV4 channels." in: Pflugers Archiv : European journal of physiology, Vol. 474, Issue 3, pp. 315-342, (2022) (PubMed).

Manneck, Manz, Braun, Rosendahl, Stumpff: "The TRPA1 Agonist Cinnamaldehyde Induces the Secretion of HCO3- by the Porcine Colon." in: International journal of molecular sciences, Vol. 22, Issue 10, (2021) (PubMed).

Liebe, Liebe, Sponder, Hedtrich, Stumpff: "Beyond Ca2+ signalling: the role of TRPV3 in the transport of NH4." in: Pflügers Archiv : European journal of physiology, (2021) (PubMed).

Liebe, Liebe, Kaessmeyer, Sponder, Stumpff: "The TRPV3 channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport." in: Pflügers Archiv : European journal of physiology, (2020) (PubMed).

Target Details for TRPV3

Target: TRPV3 (Transient Receptor Potential Cation Channel, Subfamily V, Member 3 (TRPV3))

Alternative Name: TRPV3

Background: The TRPV3 protein belongs to a family of non-selective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermo sensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. The gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels (1, 2).

Gene ID: 497948

NCBI Accession: NP_001020928

UniProt: Q4QYD9