Amyloid antibody

Catalog No. ABIN863210

The Rabbit Polyclonal anti- antibody has been validated for ELISA, IP, IHC, WB, ICC and IF. It is suitable to detect in samples from Human. There are 2+ publications available.

Quick Overview for Amyloid antibody (ABIN863210)

Target: Amyloid

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details

Specificity: Recognizes all types of amyloid oligomers. Appears to recog a peptide backbone epitope common to amyloid oligomers, but not found in native proteins, amyloidogenic monomer or mature amyloid fibrils.

Cross-Reactivity: Eukaryotes, Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Synthetic molecular mimic of soluble oligomers

Application Details

Application Notes:

• WB (1:200)
• IP (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:200 dilution of ABIN863210 was sufficient for detection of amyloid oligomers in 10 μg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References

Voth, Gwin, Francis, Balczon, Frank, Pittet, Wagener, Moser, Alexeyev, Housley, Audia, Piechocki, Madera, Simmons, Crawford, Stevens: "Virulent Pseudomonas aeruginosa infection converts antimicrobial amyloids into cytotoxic prions." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 34, Issue 7, pp. 9156-9179, (2020) (PubMed).

Epelbaum, Youssef, Lacor, Chaurand, Duplus, Brugg, Duyckaerts, Delatour: "Acute amnestic encephalopathy in amyloid-β oligomer-injected mice is due to their widespread diffusion in vivo." in: Neurobiology of aging, Vol. 36, Issue 6, pp. 2043-52, (2015) (PubMed).

Target Details

Target: Amyloid

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres (1, 2). These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease (2, 3).

Gene ID: 8666

NCBI Accession: NM_000484

UniProt: P05067