NPPA antibody (C-Term)

Catalog No. ABIN870661

This Goat Polyclonal antibody specifically detects NPPA in WB, IHC and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for NPPA antibody (C-Term) (ABIN870661)

Target: NPPA (Natriuretic Peptide A (NPPA))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NPPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-NPPA Antibody

Binding Specificity: C-Term

Purpose: Atrial natriuretic factor

Sequence: RIGAQSGLGC NSFR

Specificity: The immunizing peptide represents aa137-150 of the precursor NPPA.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RIGAQSGLGCNSFR, from the C Terminus of the protein sequence according to NP_006163.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 19 kDa band observed in Mouse and Rat Heart lysates (calculated MW of 16.4 kDa according to Human precursor NP_006163.1 and 16.6 kDa accordiong to Mouse precursor NP_032751.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NPPA

Target: NPPA (Natriuretic Peptide A (NPPA))

Alternative Name: NPPA

Background: NPPA, natriuretic peptide A, ANF, ANP, ATFB6, CDD-ANF, PND, atrial natriuretic factor, atriopeptin, cardionatrin, natriuretic peptide precursor A, prepronatriodilatin

Molecular Weight: 16.4kDa

Gene ID: 4878, 230899, 24602

NCBI Accession: NP_006163