ULK3 antibody (AA 445-458)

Catalog No. ABIN870666

This anti-ULK3 antibody is a Goat Polyclonal antibody detecting ULK3 in WB and ELISA. Suitable for Human.

Quick Overview for ULK3 antibody (AA 445-458) (ABIN870666)

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ULK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ULK3 Antibody

Binding Specificity: AA 445-458

Purpose: ULK3 (aa445-458)

Sequence: KMRESRWEAD TLDK

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KMRESRWEADTLDK, from the C Terminus of the protein sequence according to NP_001092906.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex, Hippocampus) lysates (calculated MW of 53.4 kDa according to NP_001092906.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consi

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ULK3

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Alternative Name: ULK3

Background: ULK3, unc-51-like kinase 3 (C. elegans), DKFZp434C131, FLJ90566, serine/threonine-protein kinase ULK3

Molecular Weight: 53.4kDa

Gene ID: 25989

NCBI Accession: NP_001092906