HSPG antibody

Catalog No. ABIN870827

This Mouse Monoclonal antibody specifically detects HSPG in IHC, WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HSPG antibody (ABIN870827)

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPG antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: 2E2-B5

Product Details anti-HSPG Antibody

Specificity: Monoclonal Ab 2E2/B5 is specific for a core protein epitope of a human small basement membrane heparan sulfate proteoglycan (HS-PG). mAb 2E2/B5 recognizes an epitope different from that recognized by mAb 1F10/B8 and mAb 2H7/G11. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that at least two other basement membrane HS-PG exist: Agrin, originally discovered as an important component of the neuromuscular junction, and a novel small HS-PG which was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistochemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.

Purification: Purified human small basement membrane heparan sulfate proteoglycan (HS-PG). Protein G purified.

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA (less than 1 µg/ml), Western Blot (1 µg/ml), immunohistochemistry (1 µg/ml) This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Resuspend in aqua bidest.

Buffer: 50 mM TRIS pH 7.4

Storage: 4 °C

Target Details for HSPG

Target: HSPG (Heparan Sulphate Protoglycans (HSPG))

Alternative Name: Heparan-Sulfate-Proteoglycan