MYL9 antibody (pSer20)

Catalog No. ABIN872283

This anti-MYL9 antibody is a Rabbit Polyclonal antibody detecting MYL9 in WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human and Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for MYL9 antibody (pSer20) (ABIN872283)

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MYL9 Antibody

Binding Specificity: pSer20

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Myosin Regulatory Light polypeptide 9 around the phosphorylation site of Ser20

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MYL9 antibody (ABIN872283)

Li, Sun, Yang, Hu, Jiang: "Tanshinone II a protects against lipopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway." in: Chinese journal of integrative medicine, Vol. 20, Issue 3, pp. 216-23, (2014) (PubMed).

Hoj, Davis, Fullmer, Morrell, Saguibo, Schuler, Tuttle, Hansen: "Cellular contractility changes are sufficient to drive epithelial scattering." in: Experimental cell research, Vol. 326, Issue 2, pp. 187-200, (2014) (PubMed).

Ikemura, Yamamoto, Motomura, Yamaguchi, Zhao, Iwasaki, Iwamoto: "Preventive effects of the anti-vasospasm agent via the regulation of the Rho-kinase pathway on the development of steroid-induced osteonecrosis in rabbits." in: Bone, Vol. 53, Issue 2, pp. 329-35, (2013) (PubMed).

Target Details for MYL9

Target: MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))

Alternative Name: MYL9

Background:

Synonyms: LC20, MLC2, MRLC1, MYRL2, MLC-2C, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MYL9

Background: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

Gene ID: 10398

UniProt: P24844