ZWINT antibody (AA 85-170)

Catalog No. ABIN872650

This Rabbit Polyclonal antibody specifically detects ZWINT in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for ZWINT antibody (AA 85-170) (ABIN872650)

Target: ZWINT (ZW10 Interactor (ZWINT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZWINT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ZWINT Antibody

Binding Specificity: AA 85-170

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ZWINT

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ZWINT

Target: ZWINT (ZW10 Interactor (ZWINT))

Alternative Name: ZWINT

Background:

Synonyms: HZwint1, KNTC 2 AP, KNTC2AP, MGC 117174, MGC117174, ZW10 interacting protein 1, ZW10 interactor, ZWINT 1, ZWINT1, ZWINT-1, ZWINT_HUMAN.

Background: ZWINT is a component of the MIS12 complex, which is required for kinetochore function and spindle checkpoint activity. ZWINT interacts with ZW10 and targets it to the kinetochore at prometaphase. ZWINT is detectable on the kinetochore till late anaphase. Inhibition of ZWINT expression results in chromosome missegregation, spindle checkpoint failure and, ultimately, cell death upon cytokinesis.

Gene ID: 11130