Nkx2.5/ Cardiac-Specic Homeobox 1 (AA 85-180) antibody

Catalog No. ABIN872721

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB, IF (cc), IF (p), ELISA, IHC (fro) and IHC (p). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for Nkx2.5/ Cardiac-Specic Homeobox 1 (AA 85-180) antibody (ABIN872721)

Target: Nkx2.5/ Cardiac-Specic Homeobox 1

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 85-180

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Nkx2.5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Ruan, Zhu, Yin, Chen: "Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells." in: Cytotechnology, Vol. 68, Issue 4, pp. 1257-65, (2016) (PubMed).

Target Details

Target: Nkx2.5/ Cardiac-Specic Homeobox 1

Alternative Name: Nkx2.5/Cardiac-specic homeobox 1

Background:

Synonyms: Csx, Nkx2.5, tinman, Nkx-2.5, Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, Nkx2-5, Nkx2e

Background: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

Gene ID: 18091

UniProt: P42582