Nkx2.5/ Cardiac-Specic Homeobox 1 (AA 85-180) antibody
Reactivity: Human, Mouse, Rat
WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p)
Host: Rabbit
Polyclonal
unconjugated
1 image
(1 reference)-
- Target
- Nkx2.5/ Cardiac-Specic Homeobox 1
- Binding Specificity
- AA 85-180
- Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Dog,Cow,Pig,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from mouse Nkx2.5
- Isotype
- IgG
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-
- Application Notes
-
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
-
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: "Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells." in: Cytotechnology, Vol. 68, Issue 4, pp. 1257-65, (2016) (PubMed).
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: "Inhibitor of p53-p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells." in: Cytotechnology, Vol. 68, Issue 4, pp. 1257-65, (2016) (PubMed).
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- Target
- Nkx2.5/ Cardiac-Specic Homeobox 1
- Alternative Name
- Nkx2.5/Cardiac-specic homeobox 1
- Background
-
Synonyms: Csx, Nkx2.5, tinman, Nkx-2.5, Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, Nkx2-5, Nkx2e
Background: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.
- Gene ID
- 18091
- UniProt
- P42582
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