WISP1 antibody (AA 237-295)

Catalog No. ABIN872843

The Rabbit Polyclonal anti-WISP1 antibody has been validated for ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect WISP1 in samples from Mouse. There is 1 publication available.

Quick Overview for WISP1 antibody (AA 237-295) (ABIN872843)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WISP1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-WISP1 Antibody

Binding Specificity: AA 237-295

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human WISP1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-WISP1 antibody (ABIN872843)

Xu, Zhao, Zhang, Xu, Yang, Wang, Liu: "Resveratrol Attenuates Hyperoxia-induced Oxidative Stress, Inflammation and Fibrosis and Suppresses Wnt/?-catenin Signaling in Lungs of Neonatal Rats." in: Clinical and experimental pharmacology & physiology, (2015) (PubMed).

Target Details for WISP1

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: Wisp1

Background:

Synonyms: CCN4, WISP1c, WISP1i, WISP1tc, WNT1-inducible-signaling pathway protein 1, WISP-1, CCN family member 4, Wnt-1-induced secreted protein, WISP1

Background: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.

Gene ID: 8840

UniProt: O95388

Pathways: WNT Signaling, Growth Factor Binding