PPAT antibody (AA 351-430)

Catalog No. ABIN872980

This Rabbit Polyclonal antibody specifically detects PPAT in WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for PPAT antibody (AA 351-430) (ABIN872980)

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PPAT Antibody

Binding Specificity: AA 351-430

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Glutamine PRPP amidotransferase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PPAT

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Alternative Name: Glutamine Prpp Amidotransferase

Background:

Synonyms: GPAT, PRAT, ATASE, Amidophosphoribosyltransferase, Glutamine phosphoribosylpyrophosphate amidotransferase, PPAT

Background: Phosphoribosyl pyrophosphate amidotransferase (PPAT) is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. The genes for PPAT and PAICS/AIRC, (a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway), are located in close proximity on chromosome 4.

Gene ID: 5471

UniProt: Q06203