SUPT16H antibody (AA 601-700)

Catalog No. ABIN873073

This Rabbit Polyclonal antibody specifically detects SUPT16H in ELISA, WB, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for SUPT16H antibody (AA 601-700) (ABIN873073)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SUPT16H Antibody

Binding Specificity: AA 601-700

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SUPT16H/CDC68

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background:

Synonyms: CDC68, Chromatin specic transcription elongation factor 140 kDa subunit, Chromatin-specic transcription elongation factor 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, FACT 140 kDa subunit, FACT, FACT complex subunit SPT16, FACTP140, FLJ10857, FLJ14010, hSPT16, SP16H_HUMAN, Suppressor of Ty 16 homolog, Supt16h.

Background: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Gene ID: 11198

Pathways: Chromatin Binding