HNE antibody

Catalog No. ABIN873270

This anti-HNE antibody is a Rabbit Polyclonal antibody detecting HNE in WB, ELISA, IF (p), IHC (fro) and IHC (p). Suitable for not_set. This Primary Antibody has been cited in 13+ publications.

Quick Overview for HNE antibody (ABIN873270)

Target: HNE (4-Hydroxynonenal (HNE))

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HNE Antibody

Cross-Reactivity: Monkey, Mouse, Rat

Cross-Reactivity (Details): 4-Hydroxynonenal

Purification: Purified by Protein A.

Immunogen: 4 Hydroxynonenal conjugated to BSA

Isotype: IgG

Application Details

Application Notes: IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HNE antibody (ABIN873270)

Shin, Lee, Jang, Kim, Park, Kang, Shim, Ko, Hwang: "Hemolysis-induced hepatic ferroptosis following xenotransfusion of genetically modified pig red blood cells." in: Scientific reports, Vol. 15, Issue 1, pp. 44014, (2025) (PubMed).

Zhang, Chang, Chen, Beg, Huang, Vick, Wang, Zhang, Yttre, Gupta, Castleberry, Zhang, Dai, Zhu, Song, Yang, Brown, Xu, Ma, Smith, Zielonka, Traylor, Ben Dhaou, Orr, Cui, Chen: "Oxidized LDL stimulates PKM2-mediated mtROS production and phagocytosis." in: Journal of lipid research, Vol. 66, Issue 5, pp. 100809, (2025) (PubMed).

Târtea, Popa-Wagner, Sfredel, Mitran, Dan, Țucă, Preda, Raicea, Țieranu, Cozma, Vătășescu: "Chitosan Versus Dapagliflozin in a Diabetic Cardiomyopathy Mouse Model." in: International journal of molecular sciences, Vol. 25, Issue 4, (2024) (PubMed).

Zhang, Chang, Chen, Beg, Huang, Vick, Wang, Zhang, Yttre, Gupta, Castleberry, Zhang, Dai, Song, Zhu, Yang, Brown, Xu, Ma, Smith, Zielonka, Traylor, Dhaou, Orr, Cui, Zheng, Chen: "Oxidized LDL regulates efferocytosis through the CD36-PKM2-mtROS pathway." in: bioRxiv : the preprint server for biology, (2024) (PubMed).

Chien, Wen, Cheng, Tsai, Chiang, Chien: "Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 8, (2020) (PubMed).

Vairappan, Sundhar, Srinivas: "Resveratrol Restores Neuronal Tight Junction Proteins Through Correction of Ammonia and Inflammation in CCl4-Induced Cirrhotic Mice." in: Molecular neurobiology, Vol. 56, Issue 7, pp. 4718-4729, (2019) (PubMed).

Sapkota, Gaire, Cho, Jeon, Kwon, Jang, Kim, Ryu, Choi: "Eupatilin exerts neuroprotective effects in mice with transient focal cerebral ischemia by reducing microglial activation." in: PLoS ONE, Vol. 12, Issue 2, pp. e0171479, (2017) (PubMed).

Kagaya, Sasaki, Kino, Taniguchi, Kuraishi, Andoh: "Involvement of oxidative stress in increased peripheral nerve firing during spontaneous dysesthesia in a mouse model of ischemia-reperfusion." in: Neuroscience letters, Vol. 631, pp. 109-14, (2016) (PubMed).

Zhang, Song, Pang, Zhang, Wan, Yuan, Wu, Liu: "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 14, pp. 4195-209, (2015) (PubMed).

Polverino, Doyle-Eisele, McDonald, Wilder, Royer, Laucho-Contreras, Kelly, Divo, Pinto-Plata, Mauderly, Celli, Tesfaigzi, Owen: "A novel nonhuman primate model of cigarette smoke-induced airway disease." in: The American journal of pathology, Vol. 185, Issue 3, pp. 741-55, (2015) (PubMed).

Duong, Chami, McMahon, Fong, Dennis, Freedman, Witting: "Pre-treatment with the synthetic antioxidant T-butyl bisphenol protects cerebral tissues from experimental ischemia reperfusion injury." in: Journal of neurochemistry, Vol. 130, Issue 6, pp. 733-47, (2014) (PubMed).

Haberman, Tickle, Dexheimer, Kim, Tang, Karns, Baldassano, Noe, Rosh, Markowitz, Heyman, Griffiths, Crandall, Mack, Baker, Huttenhower, Keljo, Hyams, Kugathasan, Walters, Aronow, Xavier, Gevers et al.: "Pediatric Crohn disease patients exhibit specific ileal transcriptome and microbiome signature. ..." in: The Journal of clinical investigation, Vol. 124, Issue 8, pp. 3617-33, (2014) (PubMed).

Guo, Tong, Xi, Yang, Dong, Wen: "Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats." in: Journal of ethnopharmacology, Vol. 144, Issue 3, pp. 768-74, (2012) (PubMed).

Target Details for HNE

Target: HNE (4-Hydroxynonenal (HNE))

Alternative Name: 4 Hydroxynonenal

Target Type: Chemical

Background:

Synonyms: 4-Hydroxy-2-Nonenal, 4Hydroxynonenal, 4-Hydroxynonenal, 4 HNE, 4HNE, 4-HNE, E-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA.

Background: Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress 4-HNE has been hypothesized to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion.