ILK antibody (pSer246) (AbBy Fluor® 488)

Catalog No. ABIN875042

The Rabbit Polyclonal anti-ILK antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect ILK in samples from Mouse.

Quick Overview for ILK antibody (pSer246) (AbBy Fluor® 488) (ABIN875042)

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILK antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ILK Antibody (AbBy Fluor® 488)

Binding Specificity: pSer246

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ILK-1 around the phosphorylation site of Ser246

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: ILK-1

Background:

Synonyms: ILK-1 phospho S246, ILK-1 phospho Ser246, p-ILK-1 Ser246, 59 kDa serine/threonine protein kinase, 59 kDa serine/threonine-protein kinase, ILK-2, ILK_HUMAN, Integrin linked Kinase, Integrin-linked protein kinase, DKFZp686F1765, EC 2.7.11.1, ILK 1, ILK 2, ILK, ILK1, ILK2, Integrin linked Kinase 2, Integrin linked protein kinase, p59, p59ILK.

Background: The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells, its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.

Gene ID: 3611

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration