DDX58 antibody (pThr170) (AbBy Fluor® 488)

Catalog No. ABIN877555

This Rabbit Polyclonal antibody specifically detects DDX58 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for DDX58 antibody (pThr170) (AbBy Fluor® 488) (ABIN877555)

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX58 antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-DDX58 Antibody (AbBy Fluor® 488)

Binding Specificity: pThr170

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human DDX58 around the phosphorylation site of Thr170

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DDX58

Target: DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

Alternative Name: DDX58

Background:

Synonyms: DEAD/H Asp-Glu-Ala-Asp/His box polypeptide RIG-I, DKFZp434J1111, DKFZp686N19181, FLJ13599, C330021E21, OTTHUMP00000045225, DEAD Asp-Glu-Ala-Asp box polypeptide 58, DEAD Asp Glu Ala Asp/His box polypeptide, DEAD box protein 58, Probable ATP dependent RNA helicase DDX58, Retinoic acid inducible gene 1 protein, RIG I, rig-I, RIG1, rigi, RNA helicase.

Background: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG1/DDX58) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG1/DDX58 activates the kinases TBK1 and IKKe through the adaptor protein IPS1. These kinases then phosphorylate the transcription factors IRF3 and IRF7 which are essential for the expression of type-I interferons. RIG1/DDX58 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.

Gene ID: 23586

Pathways: Activation of Innate immune Response, Hepatitis C