HIST1H1E antibody (pThr18) (AbBy Fluor® 488)

Catalog No. ABIN877572

This anti-HIST1H1E antibody is a Rabbit Polyclonal antibody detecting HIST1H1E in WB, IF (cc) and IF (p). Suitable for Mouse and Rat.

Quick Overview for HIST1H1E antibody (pThr18) (AbBy Fluor® 488) (ABIN877572)

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H1E antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-HIST1H1E Antibody (AbBy Fluor® 488)

Binding Specificity: pThr18

Specificity: This phosphorylation site is found in Rat H1C as well, but not Human or Mouse H1C. This phosphorylation site is homologous to Thr18 in Rat H1C/H1D and Mouse H1D. Due to the highly conserved nature of these histones, this antibody may react with these members when phosphorylated at this site.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HIST1H1E

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Alternative Name: Histone H1.4

Background:

Synonyms: H1E, H1.4, H1F4, H1s-4, dJ221C16.5, Histone H1.4, Histone H1b, Histone H1s-4, HIST1H1E

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Gene ID: 3008

UniProt: P10412