GIT1 antibody (pTyr510) (AbBy Fluor® 350)

Catalog No. ABIN879794

The Rabbit Polyclonal anti-GIT1 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect GIT1 in samples from Mouse.

Quick Overview for GIT1 antibody (pTyr510) (AbBy Fluor® 350) (ABIN879794)

Target: GIT1

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIT1 antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GIT1 Antibody (AbBy Fluor® 350)

Binding Specificity: pTyr510

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr510

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1

Background:

Synonyms: ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1

Background: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis, the function may involve SDCCAG3 and PTPN13 (By similarity).

Gene ID: 28964

UniProt: Q9Y2X7