Eph A3+A4+A5 (pTyr779) antibody (HRP)

Catalog No. ABIN880177

This Rabbit Polyclonal antibody specifically detects Eph A3+A4+A5 in WB, ELISA, IHC (fro) and IHC (p). It exhibits reactivity toward Mouse and Rat.

Quick Overview for Eph A3+A4+A5 (pTyr779) antibody (HRP) (ABIN880177)

Target: Eph A3+A4+A5

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: pTyr779

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of EPH 3 Tyr779

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Eph A3+A4+A5

Background:

Synonyms: EPH A3+A4+A5 phospho Y779, p-EPH A3+A4+A5 phospho Y779, CEK7, EHK 1, EHK1, EK4, EK7, EK8, EPH homology kinase 1, EPH like kinase 4, EPH like kinase 7, EPH like kinase 8, Eph like tyrosine kinase 1, EPH receptor A3, EPH receptor A4, EPH receptor A5, Ephrin type A receptor 3, Ephrin type A receptor 4, Ephrin type A receptor 5, ETK, ETK1, HEK, HEK4, HEK7, HEK8, Human embryo kinase 1, Receptor protein tyrosine kinase HEK7, Receptor protein tyrosine kinase HEK8, SEK, TYRO1, TYRO1 protein tyrosine kinase, TYRO4, TYRO4 protein tyrosine kinase, Tyrosine protein kinase receptor EHK 1, Tyrosine protein kinase receptor ETK1, Tyrosine protein kinase receptor SEK, Tyrosine protein kinase TYRO1, EPHA3_HUMAN, EPHA4_HUMAN, EPHA5_HUMAN.

Background: EphA3 is a member of the Ephrin (Eph) family. The Ephrins and Eph-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the Ephrin A (EphA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the Ephrin B (EphB) class, which are transmembrane proteins. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.

Gene ID: 2042