ALOX15B antibody (AA 13-110) (HRP)

Catalog No. ABIN882247

This Rabbit Polyclonal antibody specifically detects ALOX15B in ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for ALOX15B antibody (AA 13-110) (HRP) (ABIN882247)

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX15B antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ALOX15B Antibody (HRP)

Binding Specificity: AA 13-110

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ALOX15B/15 Lipoxygenase 2

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ALOX15B

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Alternative Name: ALOX15B

Background:

Synonyms: 15-LOX-2, Arachidonate 15-lipoxygenase B, 15-LOX-B, 15-lipoxygenase 2, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, ALOX15B

Background: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.

Gene ID: 247

UniProt: O15296