SETDB2 antibody (AA 21-120) (HRP)

Catalog No. ABIN891348

Product Details anti-SETDB2 Antibody (HRP)

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Binding Specificity: AA 21-120

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CLLD8/SETDB2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SETDB2

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Alternative Name: CLLD8 (SETDB2 Products)

Background:

Synonyms: Histone-lysine N-methyltransferase SETDB2, C13orf4, Chromosome 13 open reading frame 4, Chronic lymphocytic leukemia deletion region 8, Chronic lymphocytic leukemia deletion region gene 8, Chronic lymphocytic leukemia deletion region gene 8 protein, Clld8, CLLL8, Gm293, H3-K9-HMTase, Histone H3-K9 methyltransferase, KMT1F, Lysine N-methyltransferase 1F, Probable histone-lysine N-methyltransferase H3 lysine-9 specic, SEB2, SET domain burcated 2, SET domain protein, burcated, 2, SETB2_HUMAN, Setdb2.

Background: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.

Gene ID: 83852