FAP antibody (AA 311-410) (AbBy Fluor® 647)

Catalog No. ABIN896768

The Rabbit Polyclonal anti-FAP antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect FAP in samples from Human, Mouse and Cow. There are 2+ publications available.

Quick Overview for FAP antibody (AA 311-410) (AbBy Fluor® 647) (ABIN896768)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Reactivity: Human, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAP antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-FAP Antibody (AbBy Fluor® 647)

Binding Specificity: AA 311-410

Cross-Reactivity: Cow, Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FAPA

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-FAP antibody (AbBy Fluor® 647) (ABIN896768)

Miller, Nagy, Knapp, Laengle, Ponweiser, Groeger, Starkl, Bergmann, Wagner, Haschemi: "Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments." in: Cell metabolism, Vol. 26, Issue 5, pp. 788-800.e6, (2017) (PubMed).

Evrard, Lecce, Michelis, Nomura-Kitabayashi, Pandey, Purushothaman, dEscamard, Li, Hadri, Fujitani, Moreno, Benard, Rimmele, Cohain, Mecham, Randolph, Nabel, Hajjar, Fuster, Boehm, Kovacic: "Endothelial to mesenchymal transition is common in atherosclerotic lesions and is associated with plaque instability." in: Nature communications, Vol. 7, pp. 11853, (2016) (PubMed).

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAPA

Background:

Synonyms: FAPA, DPPIV, Prolyl endopeptidase FAP, 17 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, FAPalpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase, FAP

Background: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

Gene ID: 2191

UniProt: Q12884

Pathways: Tube Formation