PLEKHM3 antibody (AA 651-761) (HRP)

Catalog No. ABIN911436

The HRP-conjugated Rabbit Polyclonal anti-PLEKHM3 antibody (ABIN911436) specifically detects PLEKHM3 in WB, ELISA, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for PLEKHM3 antibody (AA 651-761) (HRP) (ABIN911436)

Target: PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLEKHM3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PLEKHM3 Antibody (HRP)

Binding Specificity: AA 651-761

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PLEKHM3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PLEKHM3

Target: PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))

Alternative Name: PLEKHM3

Background:

Synonyms: DAPR, PH domain containing family M member 3, PH domain-containing family M member 3, PKHM3_HUMAN, Pleckstrin homology domain containing family M member 3, Pleckstrin homology domain-containing family M member 3, PLEKHM1L, Plekhm3.

Background: PLEKHM3 (pleckstrin homology domain containing, family M, member 3), also known as DAPR or PLEKHM1L (pleckstrin homology domain containing, family M, member 1-like), is a 761 amino acid phosphoprotein that contains two pleckstrin homology (PH) domains and one phorbol-ester/DAG-type zinc finger. Conserved in chimpanzee, dog, cow, mouse, rat, chicken and zebrafish, PLEKHM3 exists as three alternatively spliced isoforms that participate in metal ion binding. The gene that encodes PLEKHM3 maps to human chromosome 2q33.3. As the second largest human chromosome, chromosome 2 makes up approximately 8 % of the human genome and contains 237 million bases encoding over 1,400 genes. Chromosome 2 contains a probable vestigial second centromere, as well as vestigial telomeres, which gives credence to the hypothesis that human chromosome 2 formed as a result of an ancient fusion of two ancestral chromosomes, which are still present in modern day apes.