Sclerostin antibody (AbBy Fluor® 555)

Catalog No. ABIN914505

This anti-Sclerostin antibody is a Rabbit Polyclonal antibody detecting Sclerostin in WB and IF (p). Suitable for Human, Mouse, Rat, Dog and Pig.

Quick Overview for Sclerostin antibody (AbBy Fluor® 555) (ABIN914505)

Target: Sclerostin (SOST)

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sclerostin antibody is conjugated to AbBy Fluor® 555

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Sclerostin Antibody (AbBy Fluor® 555)

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Sclerostin

Isotype: IgG

Application Details

Application Notes: IF(IHC-P)(1:100-500)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Sclerostin

Target: Sclerostin (SOST)

Alternative Name: Sclerostin

Background:

Synonyms: BEER, Cortical hyperostosis with syndactyly, Sclerosteosis, Sclerostin, SOST, SOST_HUMAN, VBCH.

Background: Negative regulator of bone growth.Sclerostin (SOST) is a bone morphogenetic protein (BMP) antagonist, leading to the activation of BMP signaling. It negatively regulates the formation of bone by repressing the differentiation and/or function of osteoblasts induced by BMPs. It has been shown that Sclerostin binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. The noggin-sclerostin protein complex represents a novel mechanism for the fine-tuning of BMP activity in bone homeostasis. Evidence is accumulating that one of the important mechanisms of bone regulation by sclerostin is the modulation of Wnt/Beta-catenin signaling. Sclerostin also rapidly activated ERK-1/2 MAPK signaling, indicating the involvement of additional signaling pathways.

Molecular Weight: 24-28kDa

Gene ID: 50964