SIRT4 antibody (AA 60-110) (AbBy Fluor® 350)

Catalog No. ABIN915294

This anti-SIRT4 antibody is a Rabbit Polyclonal antibody detecting SIRT4 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for SIRT4 antibody (AA 60-110) (AbBy Fluor® 350) (ABIN915294)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is conjugated to AbBy Fluor® 350

Application: Western Blotting (WB)

Product Details anti-SIRT4 Antibody (AbBy Fluor® 350)

Binding Specificity: AA 60-110

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish,Drosophila

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SIRT4/SIR 2 like protein 4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: Sirt4

Background:

Synonyms: SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4

Background: Acts both as NAD-dependent protein ADP-ribosyl transferase and NAD-dependent protein deacetylase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Down-regulates insulin secretion.

Gene ID: 23409

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion