Tissue factor antibody (AA 32-100) (AbBy Fluor® 647)

Catalog No. ABIN917849

The Rabbit Polyclonal anti-Tissue factor antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect Tissue factor in samples from Human, Mouse, Rat and Pig. There are 2+ publications available.

Quick Overview for Tissue factor antibody (AA 32-100) (AbBy Fluor® 647) (ABIN917849)

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tissue factor antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Tissue factor Antibody (AbBy Fluor® 647)

Binding Specificity: AA 32-100

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD142

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Tissue factor antibody (AbBy Fluor® 647) (ABIN917849)

Kim, Yoo, Gu, Kim: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).

Yoo, Kim, Gu, Lee, Lee, Hwang, Hwang, Kim, Kim: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).

Target Details for Tissue factor

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Alternative Name: CD142

Background:

Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3

Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Gene ID: 2152

UniProt: P13726

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling