P-Glycoprotein antibody

Catalog No. ABIN920703

This anti-P-Glycoprotein antibody is a Mouse Monoclonal antibody detecting P-Glycoprotein in WB, IHC, FACS and ICC. Suitable for Human and Hamster.

Quick Overview for P-Glycoprotein antibody (ABIN920703)

Target: P-Glycoprotein

Reactivity: Human, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Glycoprotein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: JSB-1

Product Details anti-P-Glycoprotein Antibody

Specificity: JSB-1 reacts with a conserved cytoplasmic epitope of the human plasma membrane- associated 170-180 kDa glycoprotein, the expression of which is strongly correlated with the degree of multidrug resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas.

Isotype: IgG1

Application Details

Application Notes: Can be used for the detection of MDR cells in human tumor samples. Flow Cytometry: Use at least 1:10. Fix cells in 10% (v/v) Lysing Solution, followed by primary antibody and anti-mouse-FITC. Immunocytochemistry: Use at least 1:20 (acetone fixed cell preparation) Immunohistochemistry: acetone-fixed frozen sections or formalin-fixed paraffin embedded tissues: Use at 1:20 dilution. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used, paraffin-embedded tissue can also be stained with the antibody. Western blotting: Start optimizing working dilution at 1:10, followed by incubation with anti-mouse HRP.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 ml concentrated tissue culture supernatant (200 tests) at ~250 µg IgG/ml, with 1% BSA and 0.1% NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for P-Glycoprotein

Target: P-Glycoprotein