CD44 antibody (Exon v6)

Catalog No. ABIN920730

This anti-CD44 antibody is a Mouse Monoclonal antibody detecting CD44 in FACS, WB and IHC. Suitable for Human.

Quick Overview for CD44 antibody (Exon v6) (ABIN920730)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Clone: VFF-18

Product Details anti-CD44 Antibody

Binding Specificity: Exon v6

Specificity: Specifically recognizes an epitope encoded by exon v6 on the variant portion of human CD44.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Recommended dilution is 1:20 as determined on RPMI8866 cells. Immunohistochemistry: The antibody can be used to stain both indirectly or directly acetone-fixed cryostat section or cell smears. Recommended is the alkaline phosphatase- anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. May be used at a concentration of 1-2 µg/mL for cryostat sections. Suitable for paraffin- embedded tissue after microwave treatment (1 x 5 min). May be used at a concentration of 1-2 µg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µL at 1 mg/mL antibody in PBS. Purity >95% IgG1 as determined by SDS-gel electrophoresis prior to addition of stabilizer and preservative.

Storage: -20 °C

Target Details for CD44

Target: CD44

Alternative Name: CD44

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling