T7-Tag antibody (HRP)

Catalog No. ABIN920811

The HRP-conjugated Rabbit Polyclonal anti-T7-Tag antibody (ABIN920811) specifically detects T7-Tag in WB and ELISA.

Quick Overview for T7-Tag antibody (HRP) (ABIN920811)

Target: T7-Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T7-Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-T7-Tag Antibody (HRP)

Sequence: MASMTGGQQM G

Application Details

Application Notes: Western blot: Use at 1:5,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM HEPES pH 7.0, 0.1 M sodium chloride with 0.2% BSA and 0.01% thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for T7-Tag

Target: T7-Tag

Alternative Name: T7 tag

Target Type: Tag

Background: Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support and then conjugated with horseradish peroxidase (HRP). Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. ELISA: The anti-T7 diluted 1:25,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier.