SIAH1 antibody (C-Term)

Catalog No. ABIN925895

The Rabbit Polyclonal anti-SIAH1 antibody has been validated for WB and IHC. It is suitable to detect SIAH1 in samples from Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Cow, Xenopus laevis, Chicken and Drosophila melanogaster.

Quick Overview for SIAH1 antibody (C-Term) (ABIN925895)

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Cow, Xenopus laevis, Chicken, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIAH1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Zebrafish (Brachydanio rerio), Frog, Cow (Bovine), Chicken, Fruit Fly (Drosophila melanogaster)

Immunogen: SIAH1 antibody was raised in rabbit using the C terminal of SIAH1 as the immunogen

Application Details

Application Notes: WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

SIAH1 Blocking Peptide, (ABIN5616137), is also available for use as a blocking control in assays to test for specificity of this SIAH1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 100 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for SIAH1

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Alternative Name: SIAH1

Background: SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Synonyms: Polyclonal SIAH1 antibody, Anti-SIAH1 antibody, seven in absentia homolog 1, Drosophila antibody.