Topoisomerase II alpha antibody (C-Term)

Catalog No. ABIN926005

This Rabbit Polyclonal antibody specifically detects Topoisomerase II alpha in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig and Dog.

Quick Overview for Topoisomerase II alpha antibody (C-Term) (ABIN926005)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Mouse, Rat, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: C-Term

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Pig (Porcine)

Immunogen: TOP2 A antibody was raised in rabbit using the C terminal of TOP2 as the immunogen

Application Details

Application Notes: WB: 1.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

TOP2A Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this TOP2A antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 100 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. Synonyms: Polyclonal TOP2A antibody, Anti-TOP2A antibody, topoisomerase, DNA II alpha 170kDa antibody.

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases