TRAF6 antibody (Middle Region)

Catalog No. ABIN927286

This Rabbit Polyclonal antibody specifically detects TRAF6 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig and Dog.

Quick Overview for TRAF6 antibody (Middle Region) (ABIN927286)

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRAF6 Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Pig (Porcine), Dog (Canine), Cow (Bovine)

Purification: Purified

Immunogen: TRAF6 antibody was raised in rabbit using the middle region of TRAF6 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

TRAF6 Blocking Peptide, (ABIN936268), is also available for use as a blocking control in assays to test for specificity of this TRAF6 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for TRAF6

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Alternative Name: TRAF6

Background: TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Synonyms: Polyclonal TRAF6 antibody, Anti-TRAF6 antibody, TNF receptor-associated factor 6 antibody, MGC:3310 antibody, RNF85 antibody.

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway