SNF8 antibody (N-Term)

Catalog No. ABIN927326

The Rabbit Polyclonal anti-SNF8 antibody has been validated for WB and IHC. It is suitable to detect SNF8 in samples from Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Xenopus laevis and Chicken.

Quick Overview for SNF8 antibody (N-Term) (ABIN927326)

Target: SNF8 (Vacuolar-sorting Protein SNF8 (SNF8))

Reactivity: Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Xenopus laevis, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SNF8 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Frog, Cow (Bovine), Dog (Canine), Chicken, Zebrafish (Brachydanio rerio)

Purification: Purified

Immunogen: EAP30 antibody was raised in rabbit using the N terminal of EAP30 as the immunogen

Application Details

Application Notes: Optimal conditions should be determined by the investigator.

Comment:

EAP30 Blocking Peptide, (ABIN5613289), is also available for use as a blocking control in assays to test for specificity of this EAP30 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for SNF8

Target: SNF8 (Vacuolar-sorting Protein SNF8 (SNF8))

Alternative Name: EAP30

Background: ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia. In addition to its elongation activity, ELL contains a novel type of RNA polymerase II interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. EAP30 is a subunit of the ELL complex. EAP30 can interact with ELL and derepress ELL's inhibitory activity in vitro.SNF8, VPS25, and VPS36 form ESCRT-II, a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor. Synonyms: Polyclonal EAP30 antibody, Anti-EAP30 antibodyDot3 antibody, EAP30 antibody, VPS22 antibody.