PHD1 antibody (N-Term)

Catalog No. ABIN927508

This Rabbit Polyclonal antibody specifically detects PHD1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Chicken and Xenopus laevis.

Quick Overview for PHD1 antibody (N-Term) (ABIN927508)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PHD1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Zebrafish (Brachydanio rerio), Chicken, Frog

Purification: Purified

Immunogen: EGLN2 antibody was raised in rabbit using the N terminal of EGLN2 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

EGLN2 Blocking Peptide, (ABIN5613317), is also available for use as a blocking control in assays to test for specificity of this EGLN2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2

Background: The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms. Synonyms: Polyclonal EGLN2 antibody, Anti-EGLN2 antibody, egl nine homolog 2, C. elegans antibody.

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis