OCT4 antibody (Middle Region)

Catalog No. ABIN927921

The Rabbit Polyclonal anti-OCT4 antibody (ABIN927921) specifically detects OCT4 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Pig, Dog and Rabbit samples.

Quick Overview for OCT4 antibody (Middle Region) (ABIN927921)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-OCT4 Antibody

Binding Specificity: Middle Region

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Pig (Porcine), Cow (Bovine), Dog (Canine), Rabbit

Purification: Purified

Immunogen: POU5 F1 antibody was raised in rabbit using the middle region of POU5 1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

POU5F1 Blocking Peptide, (ABIN5615478), is also available for use as a blocking control in assays to test for specificity of this POU5F1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1

Background: POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm. Synonyms: Polyclonal POU5F1 antibody, Anti-POU5F1 antibody, POU class 5 homeobox 1 antibody, MGC22487 antibody, OCT3 antibody, OCT4 antibody, OTF3 antibody, OTF4 antibody.

Pathways: Stem Cell Maintenance