ATP5F1 antibody (N-Term)

Catalog No. ABIN928241

The Rabbit Polyclonal anti-ATP5F1 antibody has been validated for WB. It is suitable to detect ATP5F1 in samples from Human and Mouse.

Quick Overview for ATP5F1 antibody (N-Term) (ABIN928241)

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP5F1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine)

Purification: Purified

Immunogen: Atp5 f1 antibody was raised in rabbit using the N terminal of Atp5 1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Atp5f1 Blocking Peptide, , is also available for use as a blocking control in assays to test for specificity of this Atp5f1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for ATP5F1

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: Atp5f1

Background: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements. Synonyms: Polyclonal Atp5f1 antibody, Anti-Atp5f1 antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 antibody, C76477 antibody.

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process