NARF antibody (Middle Region)

Catalog No. ABIN928727

The Rabbit Polyclonal anti-NARF antibody is suitable to detect NARF in samples from Human. It has been validated for WB.

Quick Overview for NARF antibody (Middle Region) (ABIN928727)

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NARF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NARF Antibody

Binding Specificity: Middle Region

Purification: Purified

Immunogen: NARF antibody was raised in rabbit using the middle region of NARF as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

NARF Blocking Peptide, (ABIN5614915), is also available for use as a blocking control in assays to test for specificity of this NARF antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for NARF

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Alternative Name: NARF

Background: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. Synonyms: Polyclonal NARF antibody, Anti-NARF antibody, nuclear prelamin A recognition factor antibody, DKFZp434G0420 antibody, FLJ10067 antibody, IOP2 antibody.