CARD8 antibody

Catalog No. ABIN930979

This Mouse Monoclonal antibody specifically detects CARD8 in WB, ICC, IP, FACS and DB. It exhibits reactivity toward Human.

Quick Overview for CARD8 antibody (ABIN930979)

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CARD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS), Dot Blot (DB)

Clone: 2108C2a

Product Details anti-CARD8 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: CARD8 antibody was raised in mouse using recombinant Human Caspase Recruitment Domain Family, Member 8 (Card8)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: CARD8 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for CARD8

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Alternative Name: CARD8

Background: The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. Synonyms: Monoclonal CARD8 antibody, Anti-CARD8 antibody, Caspase recruitment domain family antibody, DACAR antibody, NDPP1 antibody, TUCAN antibody, CARDINAL antibody, KIAA0955 antibody,MGC57162 antibody, DKFZp779L0366 antibody.

Pathways: Positive Regulation of Endopeptidase Activity