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GAPDH antibody

Name: GAPDH antibody
Brand: antibodies-online
SKU: ABIN931345
Price: 596.78 USD
Availability: InStock

GAPDH Reactivity: Human WB, ELISA, IHC, IF Host: Mouse Monoclonal 1A10 unconjugated

Western Blot showing GAPDH antibody used against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. (GAPDH antibody)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH antibody with DAB staining. (GAPDH antibody)

IHC

Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH antibody (green), showing cytoplasmic localization. (GAPDH antibody)

3 images

(6 references)

Catalog No. ABIN931345

Datasheet as PDF

Target See all GAPDH Antibodies

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Reactivity
210

144

127

63

46

39

25

25

23

19

19

19

17

15

14

14

10

9

8

6

4

4

4

4

4

4

4

3

3

2

2

2

2

1

1

1

1

1

1
Human
Host
161

83

16

11

4
Mouse
Compatible Secondaries
Clonality
184

89
Monoclonal
Conjugate
154

31

18

16

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This GAPDH antibody is un-conjugated
Application
219

101

72

65

38

34

30

26

16

15

15

15

7

6

5

4

3

3

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunogen

GAPDH antibody was raised in Mouse using a purified recombinant fragment of human GAPDH expressed in E. coli as the immunogen.

Clone

1A10

Isotype

IgG1

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Discover our top product GAPDH Primary Antibody
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Mouse Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reactivity: Human, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 233-259), (C-Term) antibody
GAPDH Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16543 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

as a liquid In ascitic fluid containg 0.03 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles

Storage

-20 °C

Storage Comment

Store at 4 °C for short term storage. Store at -20 °C for long term storage.
Wright, Wu, Dahl, Sazama, OConnell: "Nuclear localization drives ?1-adrenergic receptor oligomerization and signaling in cardiac myocytes." in: Cellular signalling, Vol. 24, Issue 3, pp. 794-802, (2012) (PubMed).

Kuroda, Ishii, Uematsu, Ohata, Coban, Akira, Aritake, Urade, Morimoto: "Silica crystals and aluminum salts regulate the production of prostaglandin in macrophages via NALP3 inflammasome-independent mechanisms." in: Immunity, Vol. 34, Issue 4, pp. 514-26, (2011) (PubMed).

Shi, Zhang, Yang, Zhang, Wei: "ROCK1 plays an essential role in the transition from cardiac hypertrophy to failure in mice." in: Journal of molecular and cellular cardiology, Vol. 49, Issue 5, pp. 819-28, (2010) (PubMed).

Wang, Hirschberg: "Diabetes-relevant regulation of cultured blood outgrowth endothelial cells." in: Microvascular research, Vol. 78, Issue 2, pp. 174-9, (2009) (PubMed).

Aligo, Jia, Manna, Konan: "Formation and function of hepatitis C virus replication complexes require residues in the carboxy-terminal domain of NS4B protein." in: Virology, Vol. 393, Issue 1, pp. 68-83, (2009) (PubMed).

von Hattingberg, Brockmeier: "The pharmacokinetic basis of optimal antibiotic dosage." in: Infection, Vol. Suppl 1, pp. 21-4, (1980) (PubMed).
Target

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name

GAPDH (GAPDH Products)