GAPDH antibody

Catalog No. ABIN931345

This Mouse Monoclonal antibody specifically detects GAPDH in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and has been mentioned in 6+ publications.

Quick Overview for GAPDH antibody (ABIN931345)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 1A10

Product Details anti-GAPDH Antibody

Immunogen: GAPDH antibody was raised in Mouse using a purified recombinant fragment of human GAPDH expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: as a liquid In ascitic fluid containg 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: -20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

References for anti-GAPDH antibody (ABIN931345)

Wright, Wu, Dahl, Sazama, OConnell: "Nuclear localization drives ?1-adrenergic receptor oligomerization and signaling in cardiac myocytes." in: Cellular signalling, Vol. 24, Issue 3, pp. 794-802, (2012) (PubMed).

Kuroda, Ishii, Uematsu, Ohata, Coban, Akira, Aritake, Urade, Morimoto: "Silica crystals and aluminum salts regulate the production of prostaglandin in macrophages via NALP3 inflammasome-independent mechanisms." in: Immunity, Vol. 34, Issue 4, pp. 514-26, (2011) (PubMed).

Shi, Zhang, Yang, Zhang, Wei: "ROCK1 plays an essential role in the transition from cardiac hypertrophy to failure in mice." in: Journal of molecular and cellular cardiology, Vol. 49, Issue 5, pp. 819-28, (2010) (PubMed).

Wang, Hirschberg: "Diabetes-relevant regulation of cultured blood outgrowth endothelial cells." in: Microvascular research, Vol. 78, Issue 2, pp. 174-9, (2009) (PubMed).

Aligo, Jia, Manna, Konan: "Formation and function of hepatitis C virus replication complexes require residues in the carboxy-terminal domain of NS4B protein." in: Virology, Vol. 393, Issue 1, pp. 68-83, (2009) (PubMed).

von Hattingberg, Brockmeier: "The pharmacokinetic basis of optimal antibiotic dosage." in: Infection, Vol. Suppl 1, pp. 21-4, (1980) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH