YARS antibody

Catalog No. ABIN933090

The Mouse Monoclonal anti-YARS antibody (Clone YAR5H08) (ABIN933090) specifically detects YARS in WB, IP, ICC, FACS and DB. The antibody is reactive with Human samples.

Quick Overview for YARS antibody (ABIN933090)

Target: YARS (Yars) (Tyrosyl-tRNA Synthetase (Yars))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This YARS antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Dot Blot (DB)

Clone: YAR5H08

Product Details anti-YARS Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: YARS antibody was raised in mouse using recombinant Tyrosyl-Trna Synthetase (Yars)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: YARS antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for YARS

Target: YARS (Yars) (Tyrosyl-tRNA Synthetase (Yars))

Alternative Name: YARS

Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. Synonyms: Monoclonal YARS antibody, Anti-YARS antibody, Tyrosyl-tRNA synthetase antibody, YRS antibody, YTS antibody, TYRRS antibody, CMTDIC antibody.