DHX38 antibody

Catalog No. ABIN933113

The Mouse Monoclonal anti-DHX38 antibody has been validated for WB, IP, FACS, ICC and DB. It is suitable to detect DHX38 in samples from Human.

Quick Overview for DHX38 antibody (ABIN933113)

Target: DHX38 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 38 (DHX38))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DHX38 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 2271C1a

Product Details anti-DHX38 Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: DHX38 antibody was raised in mouse using recombinant Human Deah (Asp-Glu-Ala-His) Box Polypeptide 38 (Dhx38)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: DHX38 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for DHX38

Target: DHX38 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 38 (DHX38))

Alternative Name: DHX38

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process. Synonyms: Monoclonal DHX38 antibody, Anti-DHX38 antibody, DEAH (Asp-Glu-Ala-His) box polypeptide 38 antibody, DDX38 antibody, PRP16 antibody, PRPF16 antibody, KIAA0224 antibody.