DNMT3B antibody

Catalog No. ABIN933161

The Mouse Monoclonal anti-DNMT3B antibody has been validated for WB, FACS, ICC and DB. It is suitable to detect DNMT3B in samples from Human.

Quick Overview for DNMT3B antibody (ABIN933161)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 2280C3a

Product Details anti-DNMT3B Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: DNMT3 B antibody was raised in mouse using recombinant Human Dna (Cytosine-5-)-Methyltransferase 3 Beta (Dnmt3 )

Isotype: IgG2b

Application Details

Application Notes: WB: 0.2-2 µg/mL, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: DNMT3 B antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: DNMT3B

Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. Synonyms: Monoclonal DNMT3B antibody, Anti-DNMT3B antibody, DNA (cytosine-5-)- methyltransferase 3 beta antibody, ICF antibody, M.HsaIIIB antibody.