GAPDH antibody

Catalog No. ABIN933343

This Mouse Monoclonal antibody specifically detects GAPDH in WB, ELISA and DB. It exhibits reactivity toward Human and has been mentioned in 12+ publications.

Quick Overview for GAPDH antibody (ABIN933343)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Clone: GA1R

Product Details anti-GAPDH Antibody

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Rabbit, Chicken, Hamster

Purification: Protein A affinity chromatography

Immunogen: GAPDH antibody was raised in mouse using recombinant GAPDH as the immunogen.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-100,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

References for anti-GAPDH antibody (ABIN933343)

Dees, Pontiggia, Jasmin, Sotgia, Lisanti, Mercier: "Essential role of STAT5a in DCIS formation and invasion following estrogen treatment." in: Aging, Vol. 12, Issue 14, pp. 15104-15120, (2020) (PubMed).

Contreras, Calderon, Varela-Ramirez, Zhang, Quan, Das, Dimmock, Skouta, Aguilera: "Induction of apoptosis via proteasome inhibition in leukemia/lymphoma cells by two potent piperidones." in: Cellular oncology (Dordrecht), Vol. 41, Issue 6, pp. 623-636, (2018) (PubMed).

Mirzamohammadi, Kozlova, Papaioannou, Paltrinieri, Ayturk, Kobayashi: "Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models." in: Nature communications, Vol. 9, Issue 1, pp. 1352, (2018) (PubMed).

Papaioannou, Mirzamohammadi, Lisse, Nishimori, Wein, Kobayashi: "MicroRNA-140 provides robustness to the regulation of hypertrophic chondrocyte differentiation by the PTHrP-HDAC4 pathway." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, (2014) (PubMed).

Wright, Wu, Dahl, Sazama, OConnell: "Nuclear localization drives ?1-adrenergic receptor oligomerization and signaling in cardiac myocytes." in: Cellular signalling, Vol. 24, Issue 3, pp. 794-802, (2012) (PubMed).

Kuroda, Ishii, Uematsu, Ohata, Coban, Akira, Aritake, Urade, Morimoto: "Silica crystals and aluminum salts regulate the production of prostaglandin in macrophages via NALP3 inflammasome-independent mechanisms." in: Immunity, Vol. 34, Issue 4, pp. 514-26, (2011) (PubMed).

Blish, Clausen, Hawkins, Garvin, Willingham, Turner, Torti, Torti: "Loss of heterozygosity and SOSTDC1 in adult and pediatric renal tumors." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 147, (2011) (PubMed).

Heineke, Wollert, Osinska, Sargent, York, Robbins, Molkentin: "Calcineurin protects the heart in a murine model of dilated cardiomyopathy." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1080-7, (2010) (PubMed).

Shi, Zhang, Yang, Zhang, Wei: "ROCK1 plays an essential role in the transition from cardiac hypertrophy to failure in mice." in: Journal of molecular and cellular cardiology, Vol. 49, Issue 5, pp. 819-28, (2010) (PubMed).

Wang, Hirschberg: "Diabetes-relevant regulation of cultured blood outgrowth endothelial cells." in: Microvascular research, Vol. 78, Issue 2, pp. 174-9, (2009) (PubMed).

Aligo, Jia, Manna, Konan: "Formation and function of hepatitis C virus replication complexes require residues in the carboxy-terminal domain of NS4B protein." in: Virology, Vol. 393, Issue 1, pp. 68-83, (2009) (PubMed).

von Hattingberg, Brockmeier: "The pharmacokinetic basis of optimal antibiotic dosage." in: Infection, Vol. Suppl 1, pp. 21-4, (1980) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling. Synonyms: Monoclonal GAPDH antibody, Anti-GAPDH antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody, GAPDH Loading Control antibody.