RFP antibody (N-Term)

Catalog No. ABIN933571

This Mouse Monoclonal antibody specifically detects RFP in WB, ELISA, IF, IHC, IP and DB. It exhibits reactivity toward Discosoma.

Quick Overview for RFP antibody (N-Term) (ABIN933571)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)

Clone: RF5R

Product Details anti-RFP Antibody

Binding Specificity: N-Term

Purification: Protein A affinity chromatography

Immunogen: RFP Tag antibody was raised in mouse using RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates as the immunogen.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-3000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Background: Red Fluorescent Protein is a protein which exhibits bright red fluorescence when exposed to blue light. Fluorescent proteins are members of a structurally homologous class of proteins that share the unique property of being self-sufficient to form a visible wavelength chromophore from a sequence of 3 amino acids within their own polypeptide sequence. It is common research practice for biologists to introduce a gene encoding an engineered fluorescent protein into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy. Synonyms: Monoclonal RFP Tag antibody, anti-RFP Tag antibody, RFP antibody, Red Fluorescent Protein antibody, RF5R antibody.