Nef antibody (FITC)

Catalog No. ABIN933664

This Mouse Monoclonal antibody specifically detects in WB and ELISA. It exhibits reactivity toward Human Immunodeficiency Virus (HIV).

Quick Overview for Nef antibody (FITC) (ABIN933664)

Target: Nef

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Western Blotting (WB), ELISA

Clone: 2H12 N-terminus

Product Details

Cross-Reactivity (Details): Reacts with recombinant nef in ELISA and WB.

Purity: > 95 % pure

Immunogen: HIV1 Nef antibody (FITC) was raised using purified recombinant nef (HIV-1) produced in E.coli expression system as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:1,000, WB: 1:1,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Supplied lyophilized in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.
Product is photosensitive and should be protected from light.
Dilute only prior to immediate use.

Storage: -80 °C

Storage Comment: Aliquot and store at -70 °C or lower.

Target Details

Target: Nef

Target Type: Viral Protein

Background: Nef is a early protein that may play a role in optimizing the host cell environment for viral replication without causing apoptosis. Nef enhances virus infectivity and pathogenicity. It down regulates surface MHC I molecules and internalized molecules are sequestered to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.  . Synonyms: Monoclonal HIV1 Nef antibody, Anti-HIV1 Nef antibody, HIV-1 Nef antibody, 3'ORF antibody, ABIN antibody, ABIN1 antibody,C terminal core protein antibody, F protein antibody, Nef antibody, Negative factor antibody, p27 antibody, VAN antibody.