Nef antibody
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- Target
- Nef
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Reactivity
- Human Immunodeficiency Virus (HIV)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), ELISA
- Purity
- > 95 % pure
- Immunogen
- HIV1 Nef antibody was raised in mouse using full length recombinant nef (HIV-1) as the immunogen.
- Clone
- 2H12
- Isotype
- IgG1
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- Application Notes
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ELISA: 1:1,000, WB: 1:1,000
Optimal conditions should be determined by the investigator. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile distilled water.
- Concentration
- Lot specific
- Buffer
- Supplied lyophilized from PBS buffer, with no preservatives added
- Preservative
- Without preservative
- Handling Advice
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -70 °C for long term storage.
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- Target
- Nef
- Synonyms
- F-protein antibody, nef protein antibody, nef antibody
- Target Type
- Viral Protein
- Background
- Nef (Negative Regulatory Factor) is a protein expressed by primate lentiviruses. These include human immunodeficiency viruses (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV). Nef is one of many pathogen-expressed proteins, known as virulence factors, which function to manipulate the host's cellular machinery and thus allow infection, survival or replication of the pathogen. Nef stands for Negative Factor. Synonyms: Monoclonal HIV1 Nef antibody, Anti-HIV1 Nef antibody, HIV-1 Nef antibody, 3'ORF antibody, ABIN antibody, ABIN1 antibody,C terminal core protein antibody, F protein antibody, Nef antibody, Negative factor antibody, p27 antibody, VAN antibody.
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