CTCF antibody (C-Term)

Catalog No. ABIN933980

The Rat Monoclonal anti-CTCF antibody has been validated for WB and IF. It is suitable to detect CTCF in samples from Mouse.

Quick Overview for CTCF antibody (C-Term) (ABIN933980)

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: KT70

Product Details anti-CTCF Antibody

Binding Specificity: C-Term

Purification: Protein G affinity chromatography

Immunogen: CTCF antibody was raised in Rat using Mouse CTCF-C-terminal fragment as the immunogen.

Isotype: IgG2b

Application Details

Application Notes: IF: 1-10 µg/mL, WB: 0.1-1 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Supplied in PBS containing 0.1 % sodium azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C/-80 °C

Storage Comment: Store at 4 °C for up to 1 month. For long term storage, aliquot and store at -20 to -80 °C.

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF

Background: The protein CTCF plays heavily in the role of repressing the insulin-like growth factor 2 gene, by binding to the H-19 imprinting control region (ICR) along with differentially-methylated region-1 (DMR1) and MAR3. Binding of targeting sequence elements by CTCF can block the interaction between enhancers and promoters, therefore limiting the activity of enhancers to certain functional domains. Besides acting as enhancer blocking, CTCF can also act as a chromatin barrier by preventing the spread of heterochromatin structures. Synonyms: Monoclonal CTCF antibody, Anti-CTCF antibody, CTC F antibody, CTC-F antibody, Transcriptional repressor CTCF antibody, 11-zinc finger protein antibody, CCCTC-binding factor antibody.