D1 Protein of PSII, (PsbA) (C-Term) antibody

Catalog No. ABIN93523

This Chicken Polyclonal antibody specifically detects D1 Protein of PSII, (PsbA) in WB. It exhibits reactivity toward Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays, Synechococcus sp., Anabaena variabilis, Triticum aestivum, Tomato, Spartina alterniflora, Petunia hybrida, Alaria esculenta, Ulva, Stylophora pistillata, Stony Coral (Turbinaria), Pinus sylvestris, Lobaria pulmonaria, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Gonyaulax polyedra, Fucus vesiculosus, Cynara cardunculus, Cyanothece, Cyanophora paradoxa, Chlamydomonas raudensis, Brachypodium sylvaticum and Amphidinium carterae. It has been mentioned in 6+ publications

Quick Overview for D1 Protein of PSII, (PsbA) (C-Term) antibody (ABIN93523)

Target: D1 Protein of PSII, (PsbA)

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays, Synechococcus sp., Anabaena variabilis, Triticum aestivum, Tomato, Spartina alterniflora, Petunia hybrida, Alaria esculenta, Ulva, Stylophora pistillata, Stony Coral (Turbinaria), Pinus sylvestris, Lobaria pulmonaria, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Gonyaulax polyedra, Fucus vesiculosus, Cynara cardunculus, Cyanothece, Cyanophora paradoxa, Chlamydomonas raudensis, Brachypodium sylvaticum, Amphidinium carterae

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: C-Term

Purpose: Anti-PsbA | D1 protein of PSII, C-terminal (chicken)

Specificity: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases. Peptide used for antibody product.

Cross-Reactivity (Details): Alaria esculenta, Amphidinium carterae, Anabaena sp., Arabidopsis thaliana, Brachypodium sylvaticum, Chlamydomonas reinhardtii, Chlamydomonas raudensis (both Antarctic and mesophilic strains), Cyanophora sp. , Cyanothece sp. ATCC 51142, Cynara cardunculus, Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petunia sp., Pinus sylvestris, Spartina alterniflora, Solanum lycopresicum, Synechococcus sp. PCC 7942, Triticum aestivum, Ulva sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Algae (brown,red),Conifers,Cryptomonads,Legumes,Stramenopiles,Euglenoids,Prochlorophytes,Xantophytes

Purification: Purified, total IgY

Immunogen: KLH-conjugated synthetic peptide derived from available plant

Isotype: IgY

Application Details

Application Notes: 1 :4000-1 : 8000, 5 μg of total protein, (WB)

Comment:

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C

Storage Comment: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Muktiono, Schulten, Heemken, Gandrass, Prange, Schnabl, Cerboncini: "Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis." in: Analytical and bioanalytical chemistry, Vol. 390, Issue 4, pp. 1195-202, (2008) (PubMed).

Garczarek, Dufresne, Blot, Cockshutt, Peyrat, Campbell, Joubin, Six: "Function and evolution of the psbA gene family in marine Synechococcus: Synechococcus sp. WH7803 as a case study." in: The ISME journal, Vol. 2, Issue 9, pp. 937-53, (2008) (PubMed).

Drath, Kloft, Batschauer, Marin, Novak, Forchhammer: "Ammonia triggers photodamage of photosystem II in the cyanobacterium Synechocystis sp. strain PCC 6803." in: Plant physiology, Vol. 147, Issue 1, pp. 206-15, (2008) (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Kuepper, Setlik, Seibert, Prasil, Setlikova, Strittmatter, Levitan, Lohscheider, Adamska, Berman-Frank: "Iron limitation in the marine cyanobacterium Trichodesmium reveals new insights into regulation of photosynthesis and nitrogen fixation." in: The New phytologist, Vol. 179, Issue 3, pp. 784-98, (2008) (PubMed).

Takahashi, Whitney, Itoh, Maruyama, Badger: "Heat stress causes inhibition of the de novo synthesis of antenna proteins and photobleaching in cultured Symbiodinium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 11, pp. 4203-8, (2008) (PubMed).

Target Details

Target: D1 Protein of PSII, (PsbA)

Background:

Background: The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.

Additional information: A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24 kDa and 16 kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands. Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies.

Molecular Weight: 38 | 28-30 kDa

Gene ID: 844802

UniProt: P83755, P0C434, Q14FH6, P14660