EXOS antibody (cleaved)

Catalog No. ABIN93557

The Chicken Polyclonal anti-EXOS antibody has been validated for WB. It is suitable to detect EXOS in samples from Pseudomonas aeruginosa. There is 1 publication available.

Quick Overview for EXOS antibody (cleaved) (ABIN93557)

Target: EXOS (ExoS Gene Product (EXOS))

Reactivity: Pseudomonas aeruginosa

Host: Chicken

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-EXOS Antibody

Binding Specificity: cleaved

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Pseudomonas aeruginosa only

Characteristics: Expected / apparent Molecular Weight of the Antigene: 48 kDa

Purification: affinity purified

Immunogen: amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Isotype: IgY

Application Details

Application Notes: 1: 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS pH 8.0+ 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.

Storage: 4 °C

Storage Comment: store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

References for anti-EXOS antibody (ABIN93557)

Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).

Target Details for EXOS

Target: EXOS (ExoS Gene Product (EXOS))

Alternative Name: Exoenzyme S (ExoS)

Background: Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Molecular Weight: 48 kDa

UniProt: Q51449