EXOS antibody (cleaved)

Catalog No. ABIN93557

There is 1 publication for this product available. The Chicken Polyclonal anti-EXOS antibody is suitable to detect EXOS in samples from Pseudomonas aeruginosa. It has been validated for WB.

Quick Overview for EXOS antibody (cleaved) (ABIN93557)

Target: EXOS (ExoS Gene Product (EXOS))

Reactivity: Pseudomonas aeruginosa

Host: Chicken

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-EXOS Antibody

Binding Specificity: cleaved

Purpose: Anti-ExoS | Exoenzyme S

Cross-Reactivity (Details): Pseudomonas aeruginosa
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Pseudomonas aeruginosa

Purification: Purified, total IgY

Immunogen: amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Isotype: IgY

Application Details

Application Notes: 1 : 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C

Storage Comment: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-EXOS antibody (ABIN93557)

Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).

Target Details for EXOS

Target: EXOS (ExoS Gene Product (EXOS))

Alternative Name: Exoenzyme S (ExoS)

Background: Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Molecular Weight: 48 kDa

UniProt: Q51449