alpha Fetoprotein antibody

Catalog No. ABIN93887

The Mouse Monoclonal anti-alpha Fetoprotein antibody has been validated for WB, ELISA, ICC, IHC (p) and RIA. It is suitable to detect alpha Fetoprotein in samples from Human.

Quick Overview for alpha Fetoprotein antibody (ABIN93887)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Clone: AFP-11

Product Details anti-alpha Fetoprotein Antibody

Purpose: Anti-alpha-Fetoprotein Purified

Specificity: The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: purified alpha-Fetoprotein

Isotype: IgG1

Application Details

Application Notes: ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: hepatocellular carcinoma, heat mediated antigen retrieval (sodium citrate buffer), mAb incubation 1 hour / RT, detection DAB.
Western blotting: Recommended dilution: 2 μg/mL, non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: alpha-Fetoprotein

Background: Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process