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CD34 antibody (FITC)

The Mouse Monoclonal anti-CD34 antibody has been validated for FACS. It is suitable to detect CD34 in samples from Human. There are 2+ publications available.
Catalog No. ABIN94079

Quick Overview for CD34 antibody (FITC) (ABIN94079)

Target

See all CD34 Antibodies
CD34

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD34 antibody is conjugated to FITC

Application

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Flow Cytometry (FACS)

Clone

4H11[APG]
  • Purpose

    Anti-Hu CD34 FITC

    Specificity

    The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

    Cross-Reactivity (Details)

    Human

    Purification

    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    Immunogen

    Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

    Isotype

    IgG1
  • Application Notes

    Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

    Restrictions

    For Research Use only
  • Reconstitution

    No reconstitution is necessary.

    Buffer

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Do not freeze.
    Avoid prolonged exposure to light.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Miksiunas, Rucinskas, Janusauskas, Labeit, Bironaite: "Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Improves Energetic Status and Cardiomyogenic Differentiation of Human Dilated Myocardium-Derived Primary Mesenchymal Cells." in: International journal of molecular sciences, Vol. 21, Issue 14, (2020) (PubMed).

    Elknerová, Lacinová, Soucek, Marinov, Stöckbauer: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, (2007) (PubMed).

  • Target

    CD34

    Alternative Name

    CD34

    Background

    CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

    Gene ID

    947

    UniProt

    P28906
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